Validation of a LC-fluorescence method for determination of free captopril in human plasma, using a pre-column derivatization reaction with monobromobimane.

@article{Tache2002ValidationOA,
  title={Validation of a LC-fluorescence method for determination of free captopril in human plasma, using a pre-column derivatization reaction with monobromobimane.},
  author={Florentin Tache and Alexandru Farca and Andrei Medvedovici and Vincent David},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2002},
  volume={28 3-4},
  pages={
          549-57
        }
}
Both derivatization of free captopril in human plasma samples using monobromobimane as fluorescent label and the corresponding HPLC-fluorescence detection (FLD) method were validated. Calibration curve for the fluorescent captopril derivative in plasma samples is linear in the ppb-ppm range with a detection limit of 4 ppb and an identification limit of 10 ppb (P%: 90; nu > or = 5). These methods were successfully applied on bioequivalence studies carried out on some marketed pharmaceutical… CONTINUE READING
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