Validation of a Clostridium Endospore Viability Assay and Analysis of Greenland Ices and Atacama Desert Soils

  title={Validation of a Clostridium Endospore Viability Assay and Analysis of Greenland Ices and Atacama Desert Soils},
  author={Wan Yang and Adrian Ponce},
  journal={Applied and Environmental Microbiology},
  pages={2352 - 2358}
  • Wan Yang, A. Ponce
  • Published 4 February 2011
  • Biology, Medicine
  • Applied and Environmental Microbiology
ABSTRACT A microscopy-based endospore viability assay (micro-EVA) capable of enumerating germinable Clostridium endospores (GCEs) in less than 30 min has been validated and employed to determine GCE concentrations in Greenland ices and Atacama Desert soils. Inoculation onto agarose doped with Tb3+ and d-alanine triggers Clostridium spore germination and the concomitant release of ∼108 molecules of dipicolinic acid (DPA) per endospore, which, under pulsed UV excitation, enables enumeration of… 
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Rapid Endospore Viability Assay of Clostridium sporogenes spores.
  • Wan Yang, A. Ponce
  • Biology, Medicine
    International journal of food microbiology
  • 2009
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The filtration-cultivation procedure, combined with long incubations, enriched for novel ultrasmall-cell isolates, which is useful for studies of their metabolic properties and mechanisms for long-term survival under extreme conditions.
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A rapid, microscopy-based endospore viability assay (μEVA) capable of enumerating germinable endospores in less than 15 min is described, validating μEVA as a rapid biodosimetry method for monitoring sterilization processes.
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