Validation of a Clostridium Endospore Viability Assay and Analysis of Greenland Ices and Atacama Desert Soils

@article{Yang2011ValidationOA,
  title={Validation of a Clostridium Endospore Viability Assay and Analysis of Greenland Ices and Atacama Desert Soils},
  author={Wan Yang and Adrian Ponce},
  journal={Applied and Environmental Microbiology},
  year={2011},
  volume={77},
  pages={2352 - 2358}
}
  • Wan Yang, A. Ponce
  • Published 4 February 2011
  • Biology, Medicine
  • Applied and Environmental Microbiology
ABSTRACT A microscopy-based endospore viability assay (micro-EVA) capable of enumerating germinable Clostridium endospores (GCEs) in less than 30 min has been validated and employed to determine GCE concentrations in Greenland ices and Atacama Desert soils. Inoculation onto agarose doped with Tb3+ and d-alanine triggers Clostridium spore germination and the concomitant release of ∼108 molecules of dipicolinic acid (DPA) per endospore, which, under pulsed UV excitation, enables enumeration of… 
Sensitive quantification of dipicolinic acid from bacterial endospores in soils and sediments
TLDR
This study presents an improved HPLC‐fluorescence method for DPA quantification using a single 10‐min run with pre‐column Tb3+ chelation that distinguishes DPA from intact and recently germinated spores, enabling responses to germinants in natural samples or experiments to be assessed in a new way.
Quantification of Endospore-Forming Firmicutes by Quantitative PCR with the Functional Gene spo0A
TLDR
An approach based on quantitative PCR has a promising potential for the detection of Firmicutes over a wide range of applications and gave a reliable quantification when tested on laboratory strains, with the exception of Sulfobacillus and Desulfotomaculum.
Estimating the Abundance of Endospores of Sulfate-Reducing Bacteria in Environmental Samples by Inducing Germination and Exponential Growth
TLDR
The method presented is a faster bioassay than most probable number enumerations and has the potential to distinguish between slow- and fast-growing SRB populations in the same sample, which presents a useful complement to other methods in ecological investigations of microbial biogeography and for specific industrial applications such as reservoir souring and corrosion risk assessments in the oil and gas sector.
Quantitative and Fast Sterility Assurance Testing of Surfaces by Enumeration of Germinable Endospores
TLDR
A 15-minute or faster assay for germinable endospores is introduced and validates to complement the conventional lengthy, culture-based surface sterility validation, which is critical in hospitals, food and pharmaceutical industries to help minimize nosocomial infection, food spoilage, and pharmaceutical contamination.
Measuring Total and Germinable Spore Populations
It has been shown that bacterial endospores can be enumerated using a microscopy based assay that images the luminescent halos from terbium ions bound to dipicolinic acid, a spore specific chemical
Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite
TLDR
Results indicate that effects of oxidizing agent treatment on subsequent spore germination are on nutrient germinant receptors in spores' inner membrane, components of the DPA release process, and the cortex‐lytic enzyme SleB, also largely in spores’ inner membrane.
A review of the abundance, behaviour and detection of clostridial pathogens in agricultural soils
The soil is a reservoir for various clostridial pathogens, with agricultural soils representing a major source of contamination for overlying crops and grazing livestock. Understanding the prevalence
Realization of Conductometry on a Digital Microfluidic Platform for Real-Time Monitoring of Bacillus Atrophaeus Endospore Germination
The advent of microelectromechanical system allows people to investigate microbial physiology in a microscale. This paper is to report the fabrication of a digital microfluidic chip coupled with
Meta-barcoded evaluation of the ISO standard 11063 DNA extraction procedure to characterize soil bacterial and fungal community diversity and composition
TLDR
The modified ISO procedure provides a better evaluation of bacterial and fungal communities and is limited to the modification of the mechanical lysis step of the existing ISO‐11063 standard, which is recommended for studies focusing on both the bacterial andFungal communities.
...
...

References

SHOWING 1-10 OF 48 REFERENCES
Rapid Endospore Viability Assay of Clostridium sporogenes spores.
  • Wan Yang, A. Ponce
  • Biology, Medicine
    International journal of food microbiology
  • 2009
Detection and Viability Assessment of Endospore-Forming Pathogens
TLDR
This chapter explores technology developments for the rapid detection, identification, and viability assessment of endospore-forming pathogens with a focus on Bacillus anthracis and their applications for detection and monitoring.
Detection and Isolation of Ultrasmall Microorganisms from a 120,000-Year-Old Greenland Glacier Ice Core
TLDR
The filtration-cultivation procedure, combined with long incubations, enriched for novel ultrasmall-cell isolates, which is useful for studies of their metabolic properties and mechanisms for long-term survival under extreme conditions.
Fast Sterility Assessment by Germinable-Endospore Biodosimetry
TLDR
A rapid, microscopy-based endospore viability assay (μEVA) capable of enumerating germinable endospores in less than 15 min is described, validating μEVA as a rapid biodosimetry method for monitoring sterilization processes.
Bacterial diversity in hyperarid Atacama Desert soils
[1] Surface and subsurface soil samples analyzed for this investigation were collected from the hyperarid Yungay region in the Atacama Desert, Chile. This report details the bacterial diversity
Endospore-Forming Bacteria: an Overview
TLDR
The classical and strict distinction between aerobic and anaerobic spore formers no longer holds; it is now known that, given the right environment, Bacillus subtilis and other Bacillus species can grow quite well anaerobically.
Phylogenetic Analysis of Anaerobic Psychrophilic Enrichment Cultures Obtained from a Greenland Glacier Ice Core
TLDR
Results show that phylogenetically diverse microorganisms have remained viable within the Greenland ice core for at least 100,000 years, and may represent a novel order or family.
Requirements for Germination of Clostridium sordellii Spores In Vitro
TLDR
The analysis of the kinetics of C. sordellii spore germination showed strong allosteric behavior in the binding of l-phenylalanine and l-alanine but not in that of bicarbonate or l-arginine, which postulated a mechanism for differential C. Sordellius spore activation in the female reproductive tract.
Chlorine Dioxide Inactivation ofCryptosporidium parvum Oocysts and Bacterial Spore Indicators
TLDR
It is suggested that future studies address issues such as oocyst purification protocols and the genetic diversity of C. parvum, since these factors might affect oocySt disinfection sensitivity.
Phylogenetic and Physiological Diversity of Microorganisms Isolated from a Deep Greenland Glacier Ice Core
TLDR
The isolation of nearly 800 aerobic organisms that were grouped by morphology and amplified rDNA restriction analysis patterns to select isolates for further study showed that at least 7 isolates represent new species within characterized genera and that 49 are different strains of known species.
...
...