Validation of SYTO 9/Propidium Iodide Uptake for Rapid Detection of Viable but Noncultivable Legionella pneumophila

@article{Gio2008ValidationOS,
  title={Validation of SYTO 9/Propidium Iodide Uptake for Rapid Detection of Viable but Noncultivable Legionella pneumophila},
  author={Maria S. Gi{\~a}o and Sandra Wilks and Nuno Filipe Azevedo and Maria Jo{\~a}o Mello Vieira and C William Keevil},
  journal={Microbial Ecology},
  year={2008},
  volume={58},
  pages={56-62}
}
Legionella pneumophila is an ubiquitous environmental microorganism that can cause Legionnaires’ disease or Pontiac fever. As a waterborne pathogen, it has been found to be resistant to chlorine disinfection and survive in drinking water systems, leading to potential outbreaks of waterborne disease. In this work, the effect of different concentrations of free chlorine was studied (0.2, 0.7, and 1.2 mg l−1), the cultivability of cells assessed by standard culture techniques (buffered charcoal… CONTINUE READING
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