Validation of RT-qPCR reference genes for in planta expression studies in Hemileia vastatrix, the causal agent of coffee leaf rust.

@article{Vieira2011ValidationOR,
  title={Validation of RT-qPCR reference genes for in planta expression studies in Hemileia vastatrix, the causal agent of coffee leaf rust.},
  author={Ana Vieira and Pedro Talhinhas and Andreia Loureiro and S{\'e}bastien Duplessis and Diana Elizabeth Fern{\'a}ndez and Marcelo Costa da Silva and Oct{\'a}vio S. Paulo and Helena Gil Azinheira},
  journal={Fungal biology},
  year={2011},
  volume={115 9},
  pages={
          891-901
        }
}
Hemileia vastatrix is a biotrophic fungus, causing coffee leaf rust in all coffee growing countries, leading to serious social and economic problems. Gene expression studies may have a key role unravelling the transcriptomics of this pathogen during interaction with the plant host. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is currently the golden standard for gene expression analysis, although an accurate normalisation is essential for adequate conclusions… CONTINUE READING
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Expression profiling of genes involved in the biotrophic colonisation of Coffea arabica leaves by Hemileia vastatrix

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  • 2011
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