Validation of DESS as a DNA Preservation Method for the Detection of Strongyloides spp. in Canine Feces

  title={Validation of DESS as a DNA Preservation Method for the Detection of Strongyloides spp. in Canine Feces},
  author={Meruyert Beknazarova and Shelby Millsteed and Gemma J Robertson and H. Whiley and K. Ross},
  journal={International Journal of Environmental Research and Public Health},
  • Meruyert Beknazarova, Shelby Millsteed, +2 authors K. Ross
  • Published 2017
  • Biology, Medicine
  • International Journal of Environmental Research and Public Health
  • Strongyloides stercoralis is a gastrointestinal parasitic nematode with a life cycle that includes free-living and parasitic forms. For both clinical (diagnostic) and environmental evaluation, it is important that we can detect Strongyloides spp. in both human and non-human fecal samples. Real-time PCR is the most feasible method for detecting the parasite in both clinical and environmental samples that have been preserved. However, one of the biggest challenges with PCR detection is DNA… CONTINUE READING
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