Validation of Analytical Methods for Plasma Total Antioxidant Capacity by Comparing with Urinary 8-Isoprostane Level.

  title={Validation of Analytical Methods for Plasma Total Antioxidant Capacity by Comparing with Urinary 8-Isoprostane Level.},
  author={Sang Gil Lee and Taoran Wang and Terrence M Vance and Patrice A Hubert and Dae-Ok Kim and Sung I. Koo and Ock K. Chun},
  journal={Journal of microbiology and biotechnology},
  volume={27 2},
  • S. LeeTaoran Wang O. Chun
  • Published 28 February 2017
  • Chemistry, Medicine
  • Journal of microbiology and biotechnology
Although several analytical methods for measuring total antioxidant capacity (TAC) have been applied to biological samples, there were often dissimilar results due to the different principles of methods applied. Thus, this study aimed to validate four conventional analytical methods for measuring plasma TAC, including the ABTS assay, DPPH assay, FRAP assay, and ORAC assay, by comparing with urinary 8-isoprostane concentration. In addition, TAC results were compared with antioxidant enzyme… 

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