Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors.

@article{Pascale2007ValidationFH,
  title={Validation for high-throughput screening of a VanRS-based reporter gene assay for bacterial cell wall inhibitors.},
  author={Gianfranco De Pascale and Comprehension Grigoriadou and Daniele Losi and Ismaela Ciciliato and M. Sosio and Stefano Donadio},
  journal={Journal of applied microbiology},
  year={2007},
  volume={103 1},
  pages={133-40}
}
AIMS The present study was undertaken to validate, for antibiotic discovery, a reporter gene assay based on a Bacillus subtilis strain expressing the Enterococcusfaecium vanRS genes and a vanH-lacZ fusion, which produced beta-galactosidase activity in the presence of cell wall inhibitors (CWI) and lysozyme. METHODS AND RESULTS The reporter assay was miniaturized, automated and validated with antibiotics and tested against portions of chemical and microbial extract libraries. The assay is… CONTINUE READING

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