Validation de la dosimétrie biologique chez des patients conditionnés par irradiation corporelle totale : étude cytogénétique conventionnelle et hybridation in situ (FISH)

  title={Validation de la dosim{\'e}trie biologique chez des patients conditionn{\'e}s par irradiation corporelle totale : {\'e}tude cytog{\'e}n{\'e}tique conventionnelle et hybridation in situ (FISH)},
  author={J Dossou and Radhia M'kacher and André Bridier and Th{\'e}odore Girinsky and Dominique Violot and {\'E}ric Lartigau and François Eschw{\`e}ge and Claude Parmentier},
  journal={Cancer Radiotherapie},
Resume Objectif. – Validation de la dosimetrie biologique par rapport a la dosimetrie physique realisee sur 15 patients atteints d'hemopathie maligne et traites par irradiation corporelle totale avant transplantation medullaire. Materiel et methodes. – La dosimetrie biologique est realisee par le denombrement des aberrations chromosomiques lymphocytaires chez un sujet, comparees a celles observees chez des volontaires sains dont les lymphocytes ont ete irradies in vitro (courbes dose–effet… Expand
Construction of dose response curves up to 6 Gy for Micronucleus and Dicentric Chromosome Aberration Assay with 6 MV X-ray Beam
Abstract In-vitro dose response curves were built with 6 MV X-ray for a dose range of 0.1–6 Gy, at a dose rate of 300 cGy/min, using Linear Accelerator (LINAC, SIMENS PRIMUS) for Cytokinesis-BlockExpand
A dose-response curve for biodosimetry from a 6 MV electron linear accelerator.
This study was designed to obtain dose calibration curves by scoring of dicentric chromosomes in peripheral blood lymphocytes irradiated in vitro with a 6 MV electron linear accelerator, which was in good agreement with published curves for similar radiation quality and dose rates. Expand
This retrospective dosimetry was performed for the victim 4 y after the accident, but the compatibility of the results obtained by physical and biological methods reinforce their validity, although in the case of partial-body exposure the biological method has limitations and demonstrates the need to find appropriate correction factors. Expand


Chromosome painting in highly irradiated Chernobyl victims: a follow-up study to evaluate the stability of symmetrical translocations and the influence of clonal aberrations for retrospective dose estimation.
Follow-up fluorescence in situ hybridization measurements of symmetrical translocations were performed in peripheral blood lymphocytes from 12 highly irradiated victims of the Chernobyl nuclear power plant accident biannually to investigate the persistence of these aberration type with time post-exposure. Expand
Fluorescence in situ hybridization detection of chromosomal aberrations in human lymphocytes: applicability to biological dosimetry.
The PAINT system of recording rearrangements was examined and the suggested numerical interpretation of this nomenclature was considered to be unsuitable for use in the estimation of dose for cases of accidental overexposure. Expand
Radiation-induced human chromosome aberrations. II. Human in vitro irradiation compared to in vitro and in vivo irradiation of marmoset leukocytes.
Whole blood cultures from humans and from the New World primate, Saguinus fuscicollis, were irradiated with various doses of 250 kV X-rays. The resulting centric ring plus dicentric aberration yieldsExpand
Radiation-induced chromosome aberrations analysed by two-colour fluorescence in situ hybridization with composite whole chromosome-specific DNA probes and a pancentromeric DNA probe.
The linear quadratic calibration curve generated for total stable translocations is based on approx. Expand
Further studies on chromosome aberration production after whole-body irradiation in man.
The dose–response curves for the production of chromosome aberrations in peripheral blood lymphocytes have been determined for radiation exposure both in vitro and in vivo, with essentially the same results after irradiation in vivo and in vitro. Expand
Validation of chromosome painting as a biodosimeter in human peripheral lymphocytes following acute exposure to ionizing radiation in vitro.
Fluorescence in situ hybridization with chromosome-specific composite DNA probes ('chromosome painting') appears to be a useful tool for quantifying symmetrical cytogenetic damage. However, aExpand
Biological indicators for radiation damage.
Methods for estimating radiation dose using biological indicators have made rapid progress during recent years. Chromosome analysis in lymphocytes still plays a central role, but it is no longer theExpand
Theoretical predictions on the equality of radiation-produced dicentrics and translocations detected by chromosome painting.
It is shown that, for a specific subset of aberrations detected by FISH, dicentric/translocation equality is predicted even at higher doses, and argued that for 'visibly complex' dicentrics or translocations, no similar equality is expected in general. Expand
Dose-response curves for simple and complex chromosome aberrations induced by X-rays and detected using fluorescence in situ hybridization.
It is suggested that simples result from lesions inducing by the same radiation track and complexes arise from the interaction of lesions induced by separate tracks. Expand
Rapid human chromosome aberration analysis using fluorescence in situ hybridization.
We have used in situ hybridization of repeat-sequence DNA probes, specific to the paracentromric locus 1q12 and the telomeric locus 1p36, to fluorescently stain regions that flank human chromosomeExpand