Validation and preliminary application of a GC-MS method for the determination of putrescine and cadaverine in the human brain: a promising technique for PMI estimation.

@article{Pelletti2019ValidationAP,
  title={Validation and preliminary application of a GC-MS method for the determination of putrescine and cadaverine in the human brain: a promising technique for PMI estimation.},
  author={Guido Pelletti and Marco Garagnani and Rossella Barone and Rafael Boscolo-Berto and Francesca Rossi and Annalisa Morotti and Raffaella Roffi and Paolo Fais and Susi Pelotti},
  journal={Forensic science international},
  year={2019},
  volume={297},
  pages={
          221-227
        }
}
View on PubMed
doi.org
10 Citations
Results Citations
1

10 Citations

Citation Type

Citation Type

CADAVERINE AND PUTRESCINE LEVELS IN HUMAN BRAIN TISSUE FOR POSTMORTEM INTERVAL ESTIMATION
TLDR
Both Cad and Put are considered valuable biomarkers for estimating the PMI, however, put as a biomarker of PMI is more accurate than Cad, as 99.5% of the variability of Put levels was due to the progressive timing of sampling, compared to that of Cad, which was only 75.2%.
Application of Innovative TGA/Chemometric Approach for Forensic Purposes: The Estimation of the Time since Death in Contaminated Specimens
TLDR
A novel approach based on thermogravimetry and chemometrics was used to estimate the time since death in the vitreous humor and to collect a databank of samples derived from postmortem examinations after medico–legal evaluation to demonstrate the possibility to correctly predict the PMI even in contaminated samples.
Optimization of cloned enzyme donor immunoassay cut-offs for drugs of abuse in post-mortem whole blood.
Postmortem submersion interval in human bodies recovered from fresh water in an area of Mediterranean climate. Application and comparison of preexisting models.
"Light cannabis" consumption in a sample of young adults: Preliminary pharmacokinetic data and psychomotor impairment evaluation.
Using vitreous humour and cerebrospinal fluid electrolytes in estimating post-mortem interval - an exploratory study
TLDR
K+ in VH is most accurate in estimating the PMI, with no significant improvement in accuracy when K+ in CSF is combined, suggesting the role of CSF electrolytes in estimating PMI may be very limited.
Developing a quantitative method to assess the decomposition of embalmed human cadavers
The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal Sepsis
TLDR
The unexpected function of host CYP1A1 in microbiota-mediated cadaverine metabolism is revealed, with crucial consequences for dysbacteriosis following MRSA-induced abdominal sepsis, indicating that inhibiting CYP 1A1 or blocking cadaversine-histamine H4 receptor signaling could be a potential therapeutic target against abdominal septicaemia.
Use of vitreous humor electrolytes in estimating postmortem interval in infant population (<1 year)
Determinação do intervalo postmordem. Ficção ou realidade

References

SHOWING 1-10 OF 37 REFERENCES
A quantitative GC-MS method for three major polyamines in postmortem brain cortex.
A quantitative method for putrescine (PUT), spermidine (SPD) and spermine (SPM) in homogenized postmortem human brain tissue is described that employs a novel, simple and rapid extractive…
Considerations regarding the validation of chromatographic mass spectrometric methods for the quantification of endogenous substances in forensics.
Decomposition chemistry of human remains: a new methodology for determining the postmortem interval.
TLDR
This study was conducted to characterize the chemistry associated with the decomposition of human remains with the objective of identifying time-dependent biomarkers of decomposition and revealed distinct patterns useful for determining the postmortem interval when based on CDHs.
Application of ethyl chloroformate derivatization for gas chromatography-mass spectrometry based metabonomic profiling.
Development of a fast and simple gas chromatographic protocol based on the combined use of alkyl chloroformate and solid phase microextraction for the assay of polyamines in human urine.
Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?
  • B. Madea
  • Chemistry
    Forensic science international
  • 2005
Does altered protein metabolism interfere with postmortem degradation analysis for PMI estimation?
TLDR
Interestingly, samples of rats recovering from muscle injury revealed additional desmin and vinculin degradation bands that did not occur in this form in any of the examined postmortem samples regardless of PMI.
Statistical evaluation of time‐dependent metabolite concentrations: estimation of post‐mortem intervals based on in situ 1H‐MRS of the brain
TLDR
A single 1H‐MRS measurement of brain tissue in situ results in PMIs with defined and favorable confidence intervals in the range of hours, thus offering a quantitative and objective method for the determination of PMIs.
Combined ion-pair extraction and gas chromatography-mass spectrometry for the simultaneous determination of diamines, polyamines and aromatic amines in Port wine and grape juice.
Comparison of post-mortem metabolic changes in sheep brain tissue in isolated heads and whole animals using 1H-MR spectroscopy—preliminary results
TLDR
The hypothesis that in a whole-body corpse, the distribution of bacteria from the gastrointestinal tract up to the brain in the late post-mortem interval can influence the metabolic decomposition of brain tissues is supported.
...
...