• DOI:10.1002/BMC.538
• Corpus ID: 35883833

Validated HPLC method for the determination of fluconazole in human plasma.

@article{Wattananat2006ValidatedHM,
  title={Validated HPLC method for the determination of fluconazole in human plasma.},
  author={Triporn Wattananat and Wiyada Akarawut},
  journal={Biomedical chromatography : BMC},
  year={2006},
  volume={20 1},
  pages={
          1-3
        }
}

• Triporn Wattananat, W. Akarawut
• Published 2006
• Chemistry
• Biomedical chromatography : BMC

A high-performance liquid chromatographic assay with UV detection was developed for the determination of fluconazole in human plasma. The method utilized solid-phase extraction for sample clean-up. The separation was performed on a C18 column by isocratic elution with a mobile phase of 10 mM acetate buffer at pH 5.0 and methanol and UV detection at 210 nm. Validation was performed according to the current recommendations of the USFDA bioanalytical method validation guidance. The method proved to be specific, accurate, precise and linear between 200 and 10,000 ng/mL with correlation coefficients greater than 0.999. The coefficient of variation was within 11% and relative deviation was less than 10%.