Validated HPLC analytical method with programmed wavelength UV detection for simultaneous determination of DRF-4367 and Phenol red in rat in situ intestinal perfusion study.

@article{Kumar2005ValidatedHA,
  title={Validated HPLC analytical method with programmed wavelength UV detection for simultaneous determination of DRF-4367 and Phenol red in rat in situ intestinal perfusion study.},
  author={T. R. Shantha Kumar and Sonia Chawla and S. K. Nachaegari and S. K. Singh and N. Srinivas},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2005},
  volume={38 1},
  pages={
          173-9
        }
}
  • T. R. Shantha Kumar, Sonia Chawla, +2 authors N. Srinivas
  • Published 2005
  • Chemistry, Medicine
  • Journal of pharmaceutical and biomedical analysis
  • A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of DRF-4367, a novel cox-2 inhibitor. The method was validated on RP C-18 analytical column. Mobile phase consisted of sodium dihydrogen orthophosphate (pH 3.2, 0.01 M)-acetonitrile-methanol (30:50:20, v… CONTINUE READING
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