Vacuole partitioning during meiotic division in yeast.

  title={Vacuole partitioning during meiotic division in yeast.},
  author={Amy D Roeder and Janet M. Shaw},
  volume={144 2},
We have examined the partitioning of the yeast vacuole during meiotic division. In pulse-chase experiments, vacuoles labeled with the lumenal ade2 fluorophore or the membrane-specific dye FM 4-64 were not inherited by haploid spores. Instead, these fluorescent markers were excluded from spores and trapped between the spore cell walls and the ascus. Serial optical sections using a confocal microscope confirmed that spores did not inherit detectable amounts of fluorescently labeled vacuoles… CONTINUE READING