Vacuolar sorting determinants within a plant storage protein trimer act cumulatively.

Abstract

The mechanism for vacuolar sorting of seed storage proteins is as yet poorly understood and no receptor has been identified to date. The homotrimeric glycoprotein phaseolin, which is the major storage protein of the common bean, requires a transient tetrapeptide at the C-terminus for its vacuolar sorting. A mutated construct without the tetrapeptide is secreted. We show here that coexpression of wild-type phaseolin and the mutated, secreted form in transgenic tobacco results in the formation of mixed trimers and partial vacuolar delivery of the mutated polypeptides and partial secretion of wild-type polypeptides. This indicates that the sorting signal has a cumulative effect within a phaseolin trimer. The result is discussed in the light of the hypothesized mechanisms for vacuolar sorting of seed storage proteins.

Statistics

050100'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

293 Citations

Semantic Scholar estimates that this publication has 293 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Holkeri2001VacuolarSD, title={Vacuolar sorting determinants within a plant storage protein trimer act cumulatively.}, author={H Holkeri and Alessandro Vitale}, journal={Traffic}, year={2001}, volume={2 10}, pages={737-41} }