Vacuolar H(+)-ATPase of adrenal secretory granules. Rapid partial purification and reconstitution into proteoliposomes.


A procedure has been developed for the rapid purification and reconstitution into phospholipid vesicles of the proton-translocating ATPase of bovine adrenal chromaffin-granule membranes. It involves fractionation of the membranes with Triton X-114, resolubilization of the ATPase with n-octyl glucoside, addition of purified lipids and removal of detergent by… (More)


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