Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin.

@article{Machesky1994VacciniaVE,
  title={Vaccinia virus expresses a novel profilin with a higher affinity for polyphosphoinositides than actin.},
  author={Laura M Machesky and Nelson B. Cole and Bernard Moss and Thomas D. Pollard},
  journal={Biochemistry},
  year={1994},
  volume={33 35},
  pages={10815-24}
}
We expressed in Escherichia coli the vaccinia virus gene for a protein similar to vertebrate profilins, purified the recombinant viral profilin, and characterized its interactions with actin and polyphosphoinositides. Compared with cellular profilins, this viral profilin has a low affinity (Kd > or = 35 microM) for human platelet actin monomers, a weak effect on the exchange of the nucleotide bound to the actin, and no detectable affinity for poly(L-proline). Vaccinia profilin binds to… CONTINUE READING

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