Vaccinia virus D4 mutants defective in processive DNA synthesis retain binding to A20 and DNA.

@article{Shudofsky2010VacciniaVD,
  title={Vaccinia virus D4 mutants defective in processive DNA synthesis retain binding to A20 and DNA.},
  author={Abigail M. Druck Shudofsky and Janice Elaine Y Silverman and Debasish Chattopadhyay and Robert P. Ricciardi},
  journal={Journal of virology},
  year={2010},
  volume={84 23},
  pages={12325-35}
}
Genome replication is inefficient without processivity factors, which tether DNA polymerases to their templates. The vaccinia virus DNA polymerase E9 requires two viral proteins, A20 and D4, for processive DNA synthesis, yet the mechanism of how this tricomplex functions is unknown. This study confirms that these three proteins are necessary and sufficient for processivity, and it focuses on the role of D4, which also functions as a uracil DNA glycosylase (UDG) repair enzyme. A series of D4… CONTINUE READING