Vaccinia Virus RNA Polymerase 6931 EXPERIMENTAL PROCEDURES Cells and Virus HeLa S 3 cells and mouse strain L fibroblasts ware grown in suspension culture in Eagle ’ s Minimal Essential Medium

A vaccinia virus-specified DNA-dependent RNA polymerase was isolated from the cytoplasm of infected HeLa cells. The enzyme appeared in cells only after infection; it differed in its chromatographic properties from all cellular RNA polymerases; and its subunit composition (seven subunits ranging in size from 13,500 to 135,000 daltons) was completely… (More)