Vaccinia Virus A6L Encodes a Virion Core Protein Required for Formation of Mature Virion

@article{Meng2006VacciniaVA,
  title={Vaccinia Virus A6L Encodes a Virion Core Protein Required for Formation of Mature Virion},
  author={Xiangzhi Meng and Addie E. Embry and Debbi Sochia and Yan Xiang},
  journal={Journal of Virology},
  year={2006},
  volume={81},
  pages={1433 - 1443}
}
ABSTRACT Vaccinia virus A6L is a previously uncharacterized gene that is conserved in all sequenced vertebrate poxviruses. Here, we constructed a recombinant vaccinia virus encoding A6 with an epitope tag and showed that A6 was expressed in infected cells after viral DNA replication and packaged in the core of the mature virion. Furthermore, we showed that A6 was essential for vaccinia virus replication by performing clustered charge-to-alanine mutagenesis on A6, which resulted in two vaccinia… 
Vaccinia Virus A6 Is a Two-Domain Protein Requiring a Cognate N-Terminal Domain for Full Viral Membrane Assembly Activity
TLDR
A6 plays an essential role not only in the formation of crescents but also in their subsequent enclosure to form immature virions, suggesting that interactions of the N domain with cognate viral proteins may be critical for virion assembly.
Vaccinia Virus A6 Is Essential for Virion Membrane Biogenesis and Localization of Virion Membrane Proteins to Sites of Virion Assembly
TLDR
Results show that A6 is an additional VACV protein that participates in an early step of virion membrane biogenesis, suggesting that MV membrane proteins or precursors of MV membranes are trafficked to sites of virions assembly through an active, virus-mediated process that requires A6.
Biogenesis of the Vaccinia Virus Membrane: Genetic and Ultrastructural Analysis of the Contributions of the A14 and A17 Proteins
TLDR
It is shown here that both proteins can associate with membranes co- but not posttranslationally, and that A17 is phosphorylated exclusively within the C-terminal tail and that this region is a direct substrate of the viral F10 kinase.
Vaccinia Virus Virion Membrane Biogenesis Protein A11 Associates with Viral Membranes in a Manner That Requires the Expression of Another Membrane Biogenesis Protein, A6
TLDR
The data showed that A11 associates with viral membranes during VACV replication, and this association requires A6 expression, and suggests that A6 regulates A11 membrane association.
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TLDR
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Association of the Vaccinia Virus A11 Protein with the Endoplasmic Reticulum and Crescent Precursors of Immature Virions
TLDR
Cell-free transcription and translation experiments indicated that both A11 and L2 are tail-anchored proteins that associate posttranslationally with membranes and likely require specific cytoplasmic targeting chaperones and may be involved in the recruitment of the ER for virus assembly.
From Crescent to Mature Virion: Vaccinia Virus Assembly and Maturation
TLDR
This review endeavors to provide an update on current knowledge of the VACV maturation processes with a specific focus on the initiation of VacV replication through to the formation of mature virions.
Direct Formation of Vaccinia Virus Membranes from the Endoplasmic Reticulum in the Absence of the Newly Characterized L2-Interacting Protein A30.5
TLDR
It is suggested that the outer surface of the poxvirus virion is derived from the luminal side of the ER membrane, similar to that of a crescents-like structure.
Structure-Function Analysis of Vaccinia Virus H7 Protein Reveals a Novel Phosphoinositide Binding Fold Essential for Poxvirus Replication
TLDR
The crystal structure of the vaccinia virus (VACV) H7 protein is determined and it is demonstrated that VACV H7 displays a novel fold for phosphoinositide binding, which is essential for poxvirus replication.
Structural basis for antagonizing a host restriction factor by C7 family of poxvirus host-range proteins
TLDR
The crystal structures of C7 and myxoma virus M64 are determined and it is found that all in the latter group of proteins bind SAMD9, indicating that diverse mammalian poxviruses use a conserved molecular claw in a C7-like protein to target S AMD9 and overcome host restriction.
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References

SHOWING 1-10 OF 28 REFERENCES
Vaccinia Virus Mutants with Alanine Substitutions in the Conserved G5R Gene Fail To Initiate Morphogenesis at the Nonpermissive Temperature
TLDR
The temperature-sensitive phenotype of the G5R mutants closely resembled the phenotypes of vaccinia virus mutants carrying conditionally lethal F10R protein kinase and H5R mutations, and F10, although required for phosphorylation of A17 and viral membrane formation, was synthesized by the G 5R mutants under nonpermissive conditions.
Repression of Vaccinia Virus Holliday Junction Resolvase Inhibits Processing of Viral DNA into Unit-Length Genomes
TLDR
Data indicated multiple roles for the vaccinia virus Holliday junction resolvase in the replication and processing of viral DNA into unit-length genomes.
Role of Vaccinia Virus A20R Protein in DNA Replication: Construction and Characterization of Temperature-Sensitive Mutants
TLDR
It is found that the A20R gene was transcribed early after infection, consistent with such a role in DNA replication, and targeted mutations were made by substituting alanines for charged amino acids occurring in 11 different clusters.
Vaccinia virus gene D8 encodes a virion transmembrane protein
TLDR
These studies demonstrated that the wild-type D8 protein was a transmembrane protein with a major extraviral domain that was released largely intact from the virus by trypsin, confirming the hypothesis that although it is associated with the virus, it is in a conformation different from that of theWild-type protein.
Clustered Charge-to-Alanine Mutagenesis of the Vaccinia Virus H5 Gene: Isolation of a Dominant, Temperature-Sensitive Mutant with a Profound Defect in Morphogenesis
TLDR
An essential role for H5 is suggested in normal virosome formation and the initiation of virion morphogenesis, as well as in genome replication and viral gene expression under nonpermissive conditions.
Genetic Analysis of the Vaccinia Virus I6 Telomere-Binding Protein Uncovers a Key Role in Genome Encapsidation
TLDR
It is proposed that the binding of I6 to viral telomeres directs genome encapsidation into the virus particle in a manner similar to that of a double-stranded virus.
Clustered Charge-to-Alanine Mutagenesis of the Vaccinia Virus A20 Gene: Temperature-Sensitive Mutants Have a DNA-Minus Phenotype and Are Defective in the Production of Processive DNA Polymerase Activity
TLDR
Data indicate that the A20 protein plays an essential role in the viral life cycle and that viruses with A20 lesions exhibit a DNA− phenotype that is correlated with a loss in processive polymerase activity as assayed in vitro.
Targeted construction of temperature-sensitive mutations in vaccinia virus by replacing clustered charged residues with alanine.
  • D. Hassett, R. Condit
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1994
TLDR
It is demonstrated that temperature-sensitive conditionally lethal mutants can be created in vaccinia virus by altering the charge characteristics of essential viral proteins.
Vaccinia Virus Proteome: Identification of Proteins in Vaccinia Virus Intracellular Mature Virion Particles
TLDR
This study provides the first comprehensive structural analysis of the infectious vaccinia virus IMV, showing that it contains 75 viral proteins, including structural proteins, enzymes, transcription factors, and predicted viral proteins not known to be expressed or present in the IMV.
...
1
2
3
...