Vaccinia-T7 RNA polymerase expression system: evaluation for the expression cloning of plasma membrane transporters.

@article{Blakely1991VacciniaT7RP,
  title={Vaccinia-T7 RNA polymerase expression system: evaluation for the expression cloning of plasma membrane transporters.},
  author={Randy D. Blakely and John David Clark and Gregory Rudnick and Susan Gaye Amara},
  journal={Analytical biochemistry},
  year={1991},
  volume={194 2},
  pages={302-8}
}
The vaccinia/T7 transient expression system, which results in rapid, high-level expression of proteins encoded by plasmids bearing T7 promoters, provides a powerful strategy for the expression cloning of membrane transporters. To test the feasibility of this approach, we introduced the rabbit Na+/glucose transporter by liposome-mediated transfection into vaccinia infected HeLa cells and determined the characteristics and sensitivity of induced [14C]alpha-methyl D-glucopyranoside uptake. We… CONTINUE READING