VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein.

@article{PrezMartn1996VTREC,
  title={VTR expression cassettes for engineering conditional phenotypes in Pseudomonas: activity of the Pu promoter of the TOL plasmid under limiting concentrations of the XylR activator protein.},
  author={Jose Manuel P{\'e}rez-Mart{\'i}n and V{\'i}ctor de Lorenzo},
  journal={Gene},
  year={1996},
  volume={172 1},
  pages={81-6}
}
A simplified procedure to construct recombinant Pseudomonas putida (Pp) and related bacteria, which transcribe conditionally specific genes inserted into their chromosome in response to lac inducers such as IPTG, has been developed. The method is based on the so-called VTR expression cassettes. These are three small (1.98-kb) DNA segments engineered as NotI restriction fragments that include a lacIq gene along with the hybrid trp/lac promoter, Ptrc, followed by an optimised translation… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Similar Papers

Loading similar papers…