VHH Phage-Based Competitive Real-Time Immuno-Polymerase Chain Reaction for Ultrasensitive Detection of Ochratoxin A in Cereal

@inproceedings{Liu2014VHHPC,
  title={VHH Phage-Based
Competitive Real-Time Immuno-Polymerase
Chain Reaction for Ultrasensitive Detection of Ochratoxin A in Cereal},
  author={Xing Liu and Yang Xu and Yong-hua Xiong and Zhui Tu and Yanping Li and Zhen-yun He and Yu-lou Qiu and Jin-heng Fu and Shirley J. Gee and Bruce D Hammock},
  booktitle={Analytical chemistry},
  year={2014}
}
Phage display-mediated immuno-polymerase chain reaction (PD-IPCR) is an ultrasensitive detection technology that combines the advantages of immuno-PCR and phage display. The phage particle, which displayed antibody fragments including single-chain fragment variable (scFv), variable domain of heavy-chain antibodies (VHH), and antigen-binding fragment (Fab) on the surface can be directly used in IPCR, supplying both the detection antibody and deoxyribonucleic acid (DNA) template. In this work, we… CONTINUE READING
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