VH gene expression and regulation in the mutant Alicia rabbit. Rescue of VHa2 allotype expression.

@article{Chen1993VHGE,
  title={VH gene expression and regulation in the mutant Alicia rabbit. Rescue of VHa2 allotype expression.},
  author={Hua Tang Chen and Cornelius B. Alexander and Glendowlyn O. Young-Cooper and Rose G. Mage},
  journal={Journal of immunology},
  year={1993},
  volume={150 7},
  pages={2783-93}
}
Rabbits of the Alicia strain, derived from rabbits expressing the VHa2 allotype, have a mutation in the H chain locus that has a cis effect upon the expression of VHa2 and VHa- genes. A small deletion at the most J-proximal (3') end of the VH locus leads to low expression of all the genes on the entire chromosome in heterozygous ali mutants and altered relative expression of VH genes in homozygotes. To study VH gene expression and regulation, we used the polymerase chain reaction to amplify the… CONTINUE READING