V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes.

@article{Lim2010VgeneAR,
  title={V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes.},
  author={Theam Soon Lim and Svetlana Mollova and Florian Rubelt and Volker Sievert and Stefan D{\"u}bel and Hans Lehrach and Zolt{\'a}n Konthur},
  journal={New biotechnology},
  year={2010},
  volume={27 2},
  pages={108-17}
}
For studying human antibody variable (V)-gene usage in any group of individuals or for the generation of recombinant human antibody libraries for phage display, quality and yield of the amplified V-gene repertoire is of utmost importance. Key parameters affecting the amplification of full antibody repertoires are V-gene specific primer design, complementary DNA (cDNA) synthesis from total RNA extracts of peripheral blood mononuclear cells (PBMCs) and ultimately the polymerase chain reaction… CONTINUE READING