V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation.

@article{Wang2003VDJRR,
  title={V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation.},
  author={Yui-Hsi Wang and Zhixin Zhang and Peter D Burrows and Hiromi Kubagawa and S. Louis Bridges and Harry W. Findley and Max D. Cooper},
  journal={Blood},
  year={2003},
  volume={101 3},
  pages={
          1030-7
        }
}
The initial B-cell repertoire is generated by combinatorial immunoglobulin V(D)J gene segment rearrangements that occur in a preferential sequence. Because cellular proliferation occurs during the course of these rearrangement events, it has been proposed that intraclonal diversification occurs during this phase of B-cell development. An opportunity to examine this hypothesis directly was provided by the identification of a human acute lymphoblastic leukemic cell line that undergoes spontaneous… 
View on PubMed
bloodjournal.org
19 Citations
Background Citations
6
Methods Citations
1

Figures from this paper

19 Citations

Citation Type

Citation Type

Contribution of Vh gene replacement to the primary B cell repertoire.
VH replacement in mice and humans.
Molecular Mechanism of Serial VH Gene Replacement
TLDR
Analyzing the molecular mechanism of serial VH replacement using a human B cell line that undergoes continuous spontaneous differentiation from pro‐B to pre‐B and then to B cell stage found that this cell line undergoes intraclonal V(D)J diversification.
Regulation of VH Replacement by B Cell Receptor–Mediated Signaling in Human Immature B Cells
TLDR
It is shown that cross-linking BCRs induces VH replacement in human EU12 μHC+ cells and in the newly emigrated immature B cells purified from peripheral blood of healthy donors or tonsillar samples.
The Development of Human B Lymphocytes
Independent recruitment of Igh alleles in V(D)J recombination
TLDR
It is demonstrated that each of the two immunoglobulin heavy chain (Igh) alleles in a B cell behaves independently of the other, up to the moment when a successful rearrangement in one allele triggers a feedback mechanism that prevents further recombination.
Partitioning of Rearranged Ig Genes by Mutation Analysis Demonstrates D-D Fusion and V Gene Replacement in the Expressed Human Repertoire1
TLDR
The establishment of objective criteria for the partitioning of sequences between VH, D, and JH gene segments has allowed us to more carefully analyze intervening putative nontemplated (N) nucleotides.
The potential role of VH replacement in editing and generating autoreactive antibodies
TLDR
Results indicated that VH replacement is essential to edit IgH genes encoding anti-SM antibodies, which are mainly contributed by the A19H heavy chain.
B-cell development in the presence of the MLL/AF4 oncoprotein proceeds in the absence of HOX A7 and HOX A9 expression
TLDR
It is suggested that B-lineage development can proceed in t(4;11) leukemic blasts in the absence of HOX-A gene expression, and microarray analysis is used to identify patterns of gene expression in BLIN-3 that may modulate MLL/AF4 oncogenesis and contribute to the retention of normal B- lineage developmental characteristics.
The molecular basis and biological significance of VH replacement
TLDR
H replacement can play a complex role in the generation of the primary B‐cell repertoire in humans through recombination‐activating gene (RAG)‐mediated recombination between a cryptic recombination signal sequence (RSS) present in almost all VH genes and the flanking 23 base pair RSS of an upstream VH gene.
...
1
2
...

References

SHOWING 1-10 OF 73 REFERENCES
Regulated progression of a cultured pre-B-cell line to the B-cell stage
TLDR
Analysis of the in vitro differentiation of 300-19 cells provides direct evidence for distinct differentiation phases of first VH and subsequently VL assembly during B-cell differentiation, and suggests that the μ heavy chain, resulting from a productive VHDJH rearrangement, has both a positive and a negative regulatory role in mediating this ordered differentiation process.
Receptor editing in B cells.
Recombination between an expressed immunoglobulin heavy-chain gene and a germline variable gene segment in a Ly 1+ B-cell lymphoma
TLDR
A rearrangement of the productive allele of NFS-5 is found to be due to a novel recombination event between VH genes which results in the replacement of most or all of the coding sequence of the initial VHQ52 rearrangements by a germline VH7183 gene.
Clonal evolution in B-lineage acute lymphoblastic leukemia by contemporaneous VH-VH gene replacements and VH-DJH gene rearrangements.
TLDR
Oligoclonal rearrangements evolved by two independent, yet seemingly contemporaneous molecular genetic mechanisms that may have relevance to the recombinatorial opportunities available during normal B-cell maturation.
Intraclonal generation of antibody mutants in germinal centres
TLDR
Direct evidence is reported for direct evidence that antigen-induced proliferation of B cells at another site, periarteriolar lymphocyte sheath-associated foci, was not associated with somatic hypermutation, and most represent cells of distinct B-cell clones which expanded locally, generating somatic antibody mutants at high rate.
Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow
TLDR
Functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr.
Fates of human B-cell precursors.
TLDR
The triad of laboratory models that mimic the bone marrow microenvironment, immunodeficiency diseases with specific defects in B- cell development, and B-lineage acute lymphoblastic leukemia can now be integrated to deepen the understanding of human B-cell development.
Establishment of a leukemic cell model for studying human pre-B to B cell differentiation.
TLDR
The BLIN-1 cell line is, to the authors' knowledge, the first defined human model for examining this critical developmental stage in human B cell ontogeny and offers a unique resource for examining variables influencing onset of kappa L chain gene rearrangement and expression.
Continuing rearrangement but absence of somatic hypermutation in immunoglobulin genes of human B cell precursor leukemia
TLDR
The data suggest that in some cases of ALL Ig heavy chain genes begin and continue to rearrange de novo within the neoplastic B cell precursor populations derived from an original malignant cell transformed at a stem cell stage of differentiation.
Immunoglobulin heavy-chain gene rearrangement in adult acute lymphoblastic leukemia reveals preferential usage of J(H)-proximal variable gene segments.
TLDR
Describing individual-segment and overall patterns of V(H) gene usage in adult B-lineage acute lymphoblastic leukemia revealed that the rate of V (H) usage is proportional to the proximity of the V( H) gene to the J(H] locus and that the relationship can be mathematically defined.
...
1
2
3
4
5
...