Utilizing bimolecular fluorescence complementation (BiFC) to assay protein-protein interaction in plants.

@article{Ohad2010UtilizingBF,
  title={Utilizing bimolecular fluorescence complementation (BiFC) to assay protein-protein interaction in plants.},
  author={Nir Ohad and Shaul Yalovsky},
  journal={Methods in molecular biology},
  year={2010},
  volume={655},
  pages={347-58}
}
Protein function is often mediated by the formation of stable or transient complexes. Here we present a method for testing protein-protein interactions in plants designated bimolecular fluorescence complementation (BiFC). The advantages of BiFC are its simplicity, reliability, and the ability to observe protein-protein interactions in different cellular compartments including membranes. BiFC is based on splitting the yellow fluorescent protein (YFP) into two nonoverlapping N-terminal (YN) and C… CONTINUE READING