Utilization of zygotic embryos of an economic rattan palm Calamus thwaitesii Becc. (Arecaceae) for somaplant regeneration and cryobanking

@inproceedings{Hemanthakumar2013UtilizationOZ,
  title={Utilization of zygotic embryos of an economic rattan palm Calamus thwaitesii Becc. (Arecaceae) for somaplant regeneration and cryobanking},
  author={Achuthan Sudarsanan Hemanthakumar and Thankappan Suvarna Preetha and Peringattulli Narayanan Krishnan and Sooriamuthu Seeni},
  booktitle={3 Biotech},
  year={2013}
}
Zygotic embryos excised from immature green fruits of the rattan palm, Calamus thwaitesii and cultured for 16 weeks under optimum culture conditions in Murashige and Skoog (MS) medium supplemented with 31.67 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 35.23 μM 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) produced mixed (compact and friable) calli at 70 and 92 % rates. The semi-friable part of the callus (~500 mg) separated and subcultured in medium containing 2.22 μM 6-benzyladenine and 1.07… CONTINUE READING

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