Utilization of spectral absorption for measurement of adenylate cyclase activity.

@article{Saegusa2000UtilizationOS,
  title={Utilization of spectral absorption for measurement of adenylate cyclase activity.},
  author={Yoshiki Saegusa and Atsushi Sugiyama and Keitaro Hashimoto},
  journal={Journal of clinical laboratory analysis},
  year={2000},
  volume={14 3},
  pages={115-9}
}
The purpose of this study was to improve our previously described enzymatic fluorometric assay of the adenylate cyclase activity. Using physicochemical characteristics of NADPH, of which a 0.1 mmol/L solution would have an optical density of 0.627, we measured the adenylate cyclase activity by the spectral absorption of NADPH. The assay consists of two parts: pharmacological modulation of adenylate cyclase and measurement of newly synthesized cyclic AMP. The latter part involves four steps… CONTINUE READING