Utilization of recombinant adenovirus and dominant negative mutants to characterize hepatocyte nuclear factor 4-regulated apolipoprotein AI and CIII expression.

@article{Fraser1997UtilizationOR,
  title={Utilization of recombinant adenovirus and dominant negative mutants to characterize hepatocyte nuclear factor 4-regulated apolipoprotein AI and CIII expression.},
  author={James D. Fraser and David R. Keller and V{\'i}ctor A. Mart{\'i}nez and D Santiso-Mere and R A Straney and Michael R Briggs},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 21},
  pages={13892-8}
}
Using recombinant adenoviral vectors and a dominant negative mutant of HNF-4, we have examined the contribution of hepatocyte nuclear factor 4 (HNF-4) to endogenous apolipoprotein AI and CIII mRNA expression. Overexpression of HNF-4 leads to a 7.4-fold increase in apolipoprotein CIII expression, while infection with the dominant negative mutant of HNF-4 reduces the level of apolipoprotein CIII mRNA by 80%, demonstrating that endogenous HNF-4 is necessary for apolipoprotein CIII expression… CONTINUE READING