Utilization of microparticles in next-generation assays for microflow cytometers.


Micron-sized particles have primarily been used in microfabricated flow cytometers for calibration purposes and proof-of-concept experiments. With increasing frequency, microparticles are serving as a platform for assays measured in these small analytical devices. Light scattering has been used to measure the agglomeration of antibody-coated particles in the presence of an antigen. Impedance detection is another technology being integrated into microflow cytometers for microparticle-based assays. Fluorescence is the most popular detection method in flow cytometry, enabling highly sensitive multiplexed assays. Finally, magnetic particles have also been used to measure antigen levels using a magnetophoretic micro-device. We review the progress of microparticle-based assays in microflow cytometry in terms of the advantages and limitations of each approach.

DOI: 10.1007/s00216-010-3848-8

Cite this paper

@article{Kim2010UtilizationOM, title={Utilization of microparticles in next-generation assays for microflow cytometers.}, author={Jason Sungtae Kim and Frances S. Ligler}, journal={Analytical and bioanalytical chemistry}, year={2010}, volume={398 6}, pages={2373-82} }