Utilization of fluorescent microspheres and a green fluorescent protein-marked strain for assessment of microbiological contamination of permafrost and ground ice core samples from the Canadian High Arctic.

@article{Juck2005UtilizationOF,
  title={Utilization of fluorescent microspheres and a green fluorescent protein-marked strain for assessment of microbiological contamination of permafrost and ground ice core samples from the Canadian High Arctic.},
  author={David F Juck and Gavin Whissell and Blaire Steven and Wayne Pollard and Christopher P McKay and Charles W Greer and Lyle G. Whyte},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 2},
  pages={1035-41}
}
Fluorescent microspheres were applied in a novel fashion during subsurface drilling of permafrost and ground ice in the Canadian High Arctic to monitor the exogenous microbiological contamination of core samples obtained during the drilling process. Prior to each drill run, a concentrated fluorescent microsphere (0.5-microm diameter) solution was applied to the interior surfaces of the drill bit, core catcher, and core tube and allowed to dry. Macroscopic examination in the field demonstrated… CONTINUE READING