Utilization of fluorescent chimeras for investigation of heterooligomeric complexes formed by human small heat shock proteins.

@article{Datskevich2012UtilizationOF,
  title={Utilization of fluorescent chimeras for investigation of heterooligomeric complexes formed by human small heat shock proteins.},
  author={Petr N. Datskevich and Evgeny V Mymrikov and Nikolai B Gusev},
  journal={Biochimie},
  year={2012},
  volume={94 8},
  pages={
          1794-804
        }
}
Fluorescent chimeras composed of enhanced cyan (or enhanced yellow) fluorescent proteins (ECFP or EYFP) and one of the four human small heat shock proteins (HspB1, HspB5, HspB6 or HspB8) were expressed in E. coli and purified. Fluorescent chimeras were used for investigation of heterooligomeric complexes formed by different small heat shock proteins (sHsp) and for analysis of their subunit exchange. EYFP-HspB1 and ECFP-HspB6 form heterooligomeric complex with apparent molecular weight of ∼280… CONTINUE READING
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