Utilization of alternative polyadenylation signals in the novel human apoptosis-inducing genehap

Abstract

hap, a novel human apoptosis-inducing gene which can interact with another newly discovered apoptosis-inducing geneASY, was identified, by cloning its cDNAs from human lung cell line (WI-38) cDNA library. Two major mRNA species (1.8 and 2.7 kb in length, respectively) were previously identified by Northern blot analysis of poly(A)+ RNA from human multiple tissues using partialhap cDNA as a probe. In the present work, the molecular mechanism accounting for the generation of the twohap transcripts were investigated. The rapid amplification of cDNA 3′-ends (3′-RACE) technique and the sequential Southern blot analysis, in conjunction with the sequencing analysis demonstrated that the twohap transcripts derive from the alternative polyadenylation site selection: a AATAAA signal at position 1 528–1 533 nt for the 1.8 kbhap mRNA: and a AATAAA signal at position 2 375–2 380 nt for the 2.7 kbhap mRNA. Furthermore, a number of regulatory elements withinhap 3′-untranslated region (3′-UTR) were also examined.

DOI: 10.1007/BF02850923

5 Figures and Tables

Cite this paper

@article{Xiaoling2008UtilizationOA, title={Utilization of alternative polyadenylation signals in the novel human apoptosis-inducing genehap}, author={Qu Xiao-ling and Qi Yi-peng and Qi - Bing}, journal={Wuhan University Journal of Natural Sciences}, year={2008}, volume={6}, pages={867-872} }