Utilization of KASP technology for wheat improvement

@article{Kaur2020UtilizationOK,
  title={Utilization of KASP technology for wheat improvement},
  author={Bhavjot Kaur and G. S. Mavi and Manpartik S. Gill and Dinesh Kumar Saini},
  journal={Cereal Research Communications},
  year={2020},
  volume={48},
  pages={409-421}
}
Hundreds of experiments involving genotyping and phenotyping of the plants are planned daily by plant researchers worldwide for wheat improvement. There are a plethora of molecular markers available that are being widely used in wheat genetic studies but have their own limitations. Developments in the areas of next-generation sequencing and bioinformatics applications have aided in the development of SNP-based markers. SNP-based assays have revolutionized the method of genotyping as these… 
Developing and deploying an efficient genotyping workflow for accelerating maize improvement in developing countries
Background: Molecular breeding is an essential tool for accelerating genetic gain in crop improvement, towards meeting the need to feed an ever-growing world population. Establishing low-cost,
Identification and Validation of High LD Hotspot Genomic Regions Harboring Stem Rust Resistant Genes on 1B, 2A (Sr38), and 7B Chromosomes in Wheat
TLDR
To select the most promising stem rust resistant genotypes, a new approach was suggested based on four criteria including, phenotypic selection, number of resistant allele(s), the genetic distance among the selected parents, and number of the different resistant alle(s) in the candidate crosses.
Identification of a novel major QTL from Chinese wheat cultivar Ji5265 for Fusarium head blight resistance in greenhouse.
KEY MESSAGE A novel major QTL for FHB resistance was mapped to a 6.8 Mb region on chromosome 2D in a Chinese wheat cultivar Ji5265, and diagnostic KASP markers were developed for detecting it in a
Modified “Allele-Specific qPCR” Method for SNP Genotyping Based on FRET
TLDR
The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments, and is estimated as two- to ten-fold cheaper than Amplifluor and KASP.
TaDrAp1 and TaDrAp2, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat
TLDR
Drought caused changes in the expression of both TaDrAp1 and TaDr Ap2 genes in opposite directions, effectively increasing expression in lower yielding cultivars, indicating an important regulatory role in plant growth, ontogenesis, and drought tolerance in bread and spelt wheat.
Combination of Marker-Assisted Backcross Selection of Yr59 and Phenotypic Selection to Improve Stripe Rust Resistance and Agronomic Performance in Four Elite Wheat Cultivars
In this study, we successfully introgressed and validated Yr59 into four elite wheat cultivars, Jimai 22, Chuanmai 42, Zhengmai 9023 and Xinmai 26 through marker-assisted backcross selection. Used as
Genomic and Transcriptomic Dissection of the Large-Effect Loci Controlling Drought-Responsive Agronomic Traits in Wheat
TLDR
Gene ontology enrichment analysis revealed that ABA-responsive genes were associated with stress-related functions in wheat and suggested previously unexpected pathways associated with abiotic stress responses in wheat.

References

SHOWING 1-10 OF 116 REFERENCES
High throughput SNP discovery and genotyping in hexaploid wheat
TLDR
The use of whole-genome resequencing data from eight wheat lines to mine for SNPs in the genic, the repetitive and non-repetitive intergenic fractions of the wheat genome, is reported on, providing the wheat community with powerful tools for both basic and applied research.
Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome‐specific KASP genotyping assays
TLDR
To identify co‐dominant SNPs, that can differentiate between heterozygotes and homozygotes, PCR‐amplified and sequenced genomic DNA from potential single‐copy regions of the wheat genome and compared them to orthologous copies from different wild relatives.
Accelerating public sector rice breeding with high-density KASP markers derived from whole genome sequencing of indica rice
TLDR
The new KASP have replaced SSRs to aid trait selection during marker-assisted backcrossing in these crosses, where target traits include rice blast and BLB resistance loci and the software is provided to provide the software for plant breeders to generate KAsP designs from their own datasets.
Genotyping-by-sequencing (GBS), an ultimate marker-assisted selection (MAS) tool to accelerate plant breeding
TLDR
Genotyping-by-sequencing (GBS) has been developed and applied in sequencing multiplexed samples that combine molecular marker discovery and genotyping and has been successfully used in implementing genome-wide association study (GWAS), genomic diversity study, genetic linkage analysis, molecular markers discovery and genomic selection under a large scale of plant breeding programs.
Single nucleotide polymorphism genotyping using Kompetitive Allele Specific PCR (KASP): overview of the technology and its application in crop improvement
TLDR
An overview of the different aspects of the KASP genotyping platform is provided, its application in crop improvement, and a comparison with the chip-based Illumina GoldenGate platform is compared.
Development and validation of KASP assays for genes underpinning key economic traits in bread wheat
TLDR
This is the first report on high-throughput screening of a large number of functional genes in a major crop and offers a robust and reliable molecular marker toolkit that can contribute towards maximizing genetic gains in wheat breeding programs.
Development and validation of a breeder-friendly KASPar marker for wheat leaf rust resistance locus Lr21
TLDR
A closed-tube, gel-free assay for high-throughput genotyping of leaf rust resistance locus Lr21 was developed and found to be effective in differentiating resistant and susceptible genotypes.
Marker Assisted Selection in Sorghum Using KASP Assay for the Detection of Single Nucleotide Polymorphism/Insertion Deletion.
TLDR
The methods for the deployment of MAS for trait introgression using endpoint genotyping technology for single nucleotide polymorphism (SNP)/insertion deletion (InDel) coupled with an application of KASP chemistry allowing for the selection of parents for generational advancement without going through the laborious and time consuming phenotypic selection and additional generations for selection of desired individuals are reported.
Exploiting the Repetitive Fraction of the Wheat Genome for High‐Throughput Single‐Nucleotide Polymorphism Discovery and Genotyping
TLDR
The development of a high‐throughput SNP discovery approach based on sequence capture of ISBP markers is reported on, and it is demonstrated that ISBP‐derived SNPs can be used in marker‐assisted breeding programs.
...
...