Utilization of [15N]glutamate by cultured astrocytes.

  title={Utilization of [15N]glutamate by cultured astrocytes.},
  author={Marc Yudkoff and Ilana B Nissim and Klaus Hummeler and Marvin S. Medow and David Pleasure},
  journal={The Biochemical journal},
  volume={234 1},
The metabolism of 0.25 mM-[15N]glutamic acid in cultured astrocytes was studied with gas chromatography-mass spectrometry. Almost all 15N was found as [2-15N]glutamine, [2-15N]glutamine, [5-15N]glutamine and [15N]alanine after 210 min of incubation. Some incorporation of 15N into aspartate and the 6-amino position of the adenine nucleotides also was observed, the latter reflecting activity of the purine nucleotide cycle. After the addition of [15N]glutamate the ammonia concentration in the… CONTINUE READING
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