Utility of universal sample processing methodology, combining smear microscopy, culture, and PCR, for diagnosis of pulmonary tuberculosis.

@article{Chakravorty2005UtilityOU,
  title={Utility of universal sample processing methodology, combining smear microscopy, culture, and PCR, for diagnosis of pulmonary tuberculosis.},
  author={Soumitesh Chakravorty and Mridu Dudeja and Mohd Hanif and Jaya Sivaswami Tyagi},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 6},
  pages={
          2703-8
        }
}
The universal sample processing (USP) multipurpose methodology was developed for the diagnosis of tuberculosis (TB) and other mycobacterial diseases by using smear microscopy, culture, and PCR (S. Chakravorty and J. S. Tyagi, J. Clin. Microbiol. 43:2697-2702, 2005). Its performance was evaluated in a blinded study of 571 sputa and compared with that of the direct and N-acetyl L-cysteine (NALC)-NaOH methods of smear microscopy and culture. With culture used as the gold standard, USP smear… CONTINUE READING

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