• Corpus ID: 2669097

Utilisation Of Spirulinasp. And Chlorellapyrenoidosa Biomass For The Productionof Enzymatic Protein Hydrolysates

@inproceedings{Lisba2014UtilisationOS,
  title={Utilisation Of Spirulinasp. And Chlorellapyrenoidosa Biomass For The Productionof Enzymatic Protein Hydrolysates},
  author={Cristiane Reinaldo Lisb{\^o}a and Aline Massia Pereira and Shana Pires Ferreira and A Jorge},
  year={2014}
}

• C. Lisbôa, Aline Massia Pereira, +1 author A. Jorge
• Published 2014
• Biology

This aim of this study was to assess the hydrolysis reaction of the biomass of Chlorella pyrenoidosaandSpirulinasp. LEB 18,using commercial proteases that act in different pH ranges, to obtain protein hydrolysates with promising application in food or food supplement, improving functional and nutritional food properties. Threecentral composite study designs were carried out for each microalga (Chlorella and Spirulina). The 2 3 type central composite design was utilized with three replications at the central point, varying the enzyme concentration (5 to 10 U.mL -1 ), the concentrationof substrate (5 to 10 %) and reaction time (60 to 240 min), for a total of 11 experiments per planning. The highestdegrees of hydrolysis (52.9% and 55.31%) forSpirulinaand Chlorella,respectively, were obtained with 4 h of reaction. The results show that it is possible to obtain enzymatic protein hydrolysates with different DH from microalgae biomass. Keywords- experimental design; enzymatic hydrolysis,microalgae, proteases