Using three-dimensional acinar structures for molecular and cell biological assays.

@article{Xiang2006UsingTA,
  title={Using three-dimensional acinar structures for molecular and cell biological assays.},
  author={Bin Xiang and Senthil K Muthuswamy},
  journal={Methods in enzymology},
  year={2006},
  volume={406},
  pages={692-701}
}
Epithelial cells grown in three-dimensional matrix allows one to investigate biological processes in a setting that is closer to in vivo conditions than those obtained by growing cells on plastic culture dishes. Here we outline procedures that allow one to investigate molecular mechanisms that regulate formation and disruption of three-dimensional epithelial structures. 

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