Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis.

@article{Pearce2005UsingTP,
  title={Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis.},
  author={Callum B. Pearce and Vitaly Zinkevich and Iwona B. Beech and Viera Funjika and Ana Mar{\'i}a Garc{\'i}a Ru{\'i}z and Afraa Aladawi and Heather P Duncan},
  journal={World journal of gastroenterology},
  year={2005},
  volume={11 45},
  pages={
          7142-7
        }
}
AIM To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP. METHODS Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE II score > or = 8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d 1, 3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical… CONTINUE READING
BETA