Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila

@article{Cochem2012UsingTM,
  title={Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila},
  author={Helena M. Cochem{\'e} and Angela Logan and Tracy A. Prime and Irina V. Abakumova and Caroline Quin and Stephen J. McQuaker and Jigna V. Patel and Ian M. Fearnley and Andrew M. James and Carolyn M. Porteous and Robin A. J. Smith and Richard C Hartley and Linda Partridge and M. L. Prato Murphy},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={946-958}
}
The role of hydrogen peroxide (H2O2) in mitochondrial oxidative damage and redox signaling is poorly understood, because it is difficult to measure H2O2 in vivo. Here we describe a method for assessing changes in H2O2 within the mitochondrial matrix of living Drosophila. We use a ratiometric mass spectrometry probe, MitoB ((3-hydroxybenzyl)triphenylphosphonium bromide), which contains a triphenylphosphonium cation component that drives its accumulation within mitochondria. The arylboronic… CONTINUE READING

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implications for mitochondria-specific therapies and probes

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