Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution

@article{Gillings2015UsingTC,
  title={Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution},
  author={M. Gillings and W. Gaze and A. Pruden and K. Smalla and J. Tiedje and Yong-Guan Zhu},
  journal={The ISME Journal},
  year={2015},
  volume={9},
  pages={1269 - 1279}
}
Around all human activity, there are zones of pollution with pesticides, heavy metals, pharmaceuticals, personal care products and the microorganisms associated with human waste streams and agriculture. This diversity of pollutants, whose concentration varies spatially and temporally, is a major challenge for monitoring. Here, we suggest that the relative abundance of the clinical class 1 integron-integrase gene, intI1, is a good proxy for pollution because: (1) intI1 is linked to genes… Expand
DNA as a Pollutant: the Clinical Class 1 Integron
TLDR
The clinical class 1 integron has become spectacularly successful over a very short period of time because it has a unique set of properties and prospers under anthropogenic disturbance and is disseminated in vast numbers by virtue of colonizing humans and agricultural animals. Expand
The Prevalence of Integrons as the Carrier of Antibiotic Resistance Genes in Natural and Man-Made Environments.
TLDR
This study established the pipeline for broad monitoring of intI1 in various environmental samples and scanning the ARGs carried by integrons, and supplemented knowledge on the distribution of class 1 integrons and ARG carried on mobile genetic elements, benefiting future studies on horizontal gene transfer of ARGs. Expand
Clinical class 1 integron-integrase gene - A promising indicator to monitor the abundance and elimination of antibiotic resistance genes in an urban wastewater treatment plant.
TLDR
Clinical intI1 can be adopted as an indicator for the abundance and removal efficiency of ARGs in the WWTP, as it remained rather consistent proportion with the ARG abundance in the AS and permeate, was stronger correlated with human pathogens, and was associated with greater number of ARG over time. Expand
Diversity of gene cassettes and the abundance of the class 1 integron-integrase gene in sediment polluted by metals
TLDR
It is demonstrated that most of the gene cassettes have no ascribable function and, apparently, historically metal-contaminated sediment favors the maintenance of bacteria containing the intI1 gene. Expand
Human Activity Determines the Presence of Integron-Associated and Antibiotic Resistance Genes in Southwestern British Columbia
TLDR
It is shown that ARGs were driven mainly by environmental factors from anthropogenized sites in agriculture and urban watersheds, and the high richness and gene copy numbers in impacted sites suggest that the effects of effluents on microbial communities are occurring even at low concentrations of antimicrobials in the water column. Expand
Suspended Materials in River Waters Differentially Enrich Class 1 Integron- and IncP-1 Plasmid-Carrying Bacteria in Sediments
TLDR
Plasmid-mediated adhesion to particles is proposed to be one of the main contributors in the formation of mobile genetic element-reservoirs in sediments, withAdhesion to suspended matter working as a selective enrichment process of antibiotic resistant genes and bacteria. Expand
Evolution of class 1 integrons: Mobilization and dispersal via food-borne bacteria
TLDR
It is suggested that the qacE gene cassette, conferring resistance to biocides, together with the mercury resistance operon carried by Tn21, provided a selective advantage when this bacterium made its way into the human commensal flora via food and supports the hypothesis that the ancestral class 1 integron migrated into anthropogenic settings via foodstuffs. Expand
Co-occurrence of integrase 1, antibiotic and heavy metal resistance genes in municipal wastewater treatment plants.
TLDR
The results suggest a potentially crucial role of HMRGs in the spread, mediated by mobile elements, of some ARGs, i.e. sulII and heavy metal resistance genes (HMRGs) and of a mobile element (the class I integron) in three WWTPs. Expand
Continental-scale pollution of estuaries with antibiotic resistance genes
TLDR
The strong correlations of identified resistance genes with known mobile elements, network analyses and partial redundancy analysis all led to the conclusion that human activity is responsible for the abundance and dissemination of these ARGs. Expand
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes.
TLDR
This protocol details a streamlined approach for detecting class 1 integrons and their associated resistance gene cassettes in foodstuffs, using culturing and PCR. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 119 REFERENCES
Integron Gene Cassettes and Degradation of Compounds Associated with Industrial Waste: The Case of the Sydney Tar Ponds
TLDR
It is shown that a heavily impacted estuary, exposed for almost a century to products of coal and steel industries, has developed a rich and unique cassette metagenome, containing genes likely to aid in the catabolism of compounds associated with industrial waste found there. Expand
Impacts of anthropogenic activity on the ecology of class 1 integrons and integron-associated genes in the environment
TLDR
It is shown that prevalence of class 1 integrons is higher in bacteria exposed to detergents and/or antibiotic residues, specifically in sewage sludge and pig slurry compared with agricultural soils to which these waste products are amended. Expand
Class 1 Integrons in Environments with Different Degrees of Urbanization
TLDR
The high frequency of intI 1 pseudogenes suggests that, although intI1 has a deleterious impact within several genomes, it can easily be disseminated among natural bacterial communities. Expand
Class 1 Integrons Potentially Predating the Association with Tn402-Like Transposition Genes Are Present in a Sediment Microbial Community
ABSTRACT Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread ofExpand
Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis
TLDR
In this study, insights are provided into the occurrence of genes typically associated with the class 1 integrons in previously not studied environments with or without human impact and their association with IncP-1 plasmids. Expand
The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance
TLDR
It is shown that class 1 integrons can be found on the chromosomes of nonpathogenic soil and freshwater Betaproteobacteria, and it is concluded that environmental Betaproteinobacteria were the original source of these genetic elements. Expand
Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.
TLDR
It was shown that the abundance of the intI1 gene in creek sediment correlates with ecological condition, implying that class 1 integrons provide selective advantages relevant to environmental pressures other than the use of antibiotics. Expand
Occurrence, abundance and elimination of class 1 integrons in one municipal sewage treatment plant
TLDR
Class 1 integrons are wide-spread in STPs which might be involved in multiple resistances in the activated sludge characterized by high biomass and biodiversity. Expand
Evolutionary consequences of antibiotic use for the resistome, mobilome and microbial pangenome
TLDR
The human use and environmental release of antibiotics is having second order effects on the microbial world, because these small molecules act as drivers of bacterial evolution. Expand
Influence of industrial contamination on mobile genetic elements: class 1 integron abundance and gene cassette structure in aquatic bacterial communities
TLDR
It is determined that the gene cassette pool is a novel and diverse resource available for bacterial acquisition, but that contamination has no discernible effect on cassette richness, and class 1 integrons are ubiquitous and abundant in environmental bacterial communities. Expand
...
1
2
3
4
5
...