Using size exclusion chromatography‐RPLC and RPLC‐CIEF as two‐dimensional separation strategies for protein profiling

  title={Using size exclusion chromatography‐RPLC and RPLC‐CIEF as two‐dimensional separation strategies for protein profiling},
  author={David C. Simpson and Seonghee Ahn and Ljiljana Pa{\vs}a-Toli{\'c} and Bogdan Bogdanov and Heather M. Mottaz and Andrey N. Vilkov and Gordon A. Anderson and Mary S. Lipton and Richard D. Smith},
Bottom‐up proteomics (analyzing peptides that result from protein digestion) has demonstrated capability for broad proteome coverage and good throughput. However, due to incomplete sequence coverage, this approach is not ideally suited to the study of modified proteins. The modification complement of a protein can best be elucidated by analyzing the intact protein. 2‐DE, typically coupled with the analysis of peptides that result from in‐gel digestion, is the most frequently applied protein… 
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