Using quantitative real-time PCR to determine the hygienic status of cattle manure.

@article{Lebuhn2003UsingQR,
  title={Using quantitative real-time PCR to determine the hygienic status of cattle manure.},
  author={Michael Lebuhn and Martin Effenberger and Andreas Gronauer and Peter A. Wilderer and Stefan Wuertz},
  journal={Water science and technology : a journal of the International Association on Water Pollution Research},
  year={2003},
  volume={48 4},
  pages={97-103}
}
We developed a suitable system of DNA extraction and real-time quantitative polymerase chain reaction (qPCR) for the specific and sensitive quantification of pathogens and other relevant (indicator) organisms in recalcitrant material such as cattle manure. PCR inhibition by coextraction of humic compounds was minimized in this system, resulting in detection sensitivity of one target DNA copy per reaction well. Data from qPCR analysis for Escherichia coli agreed with cultivation based results… CONTINUE READING

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