Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences.

@article{Wittkopp2011UsingPT,
  title={Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences.},
  author={Patricia J. Wittkopp},
  journal={Methods in molecular biology},
  year={2011},
  volume={772},
  pages={
          297-317
        }
}
  • P. Wittkopp
  • Published 2011
  • Biology
  • Methods in molecular biology
Changes in gene expression are an important source of phenotypic differences within and between species. Differences in RNA abundance can be readily quantified between genotypes using a variety of tools, including microarrays, quantitative real-time PCR, cDNA sequencing, and in situ hybridization, but determining the genetic basis of heritable expression differences has historically been less straightforward. Genetic changes that affect RNA abundance can be broadly classified into two groups… 
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