Using next generation sequencing to identify yellow fever virus in Uganda.

  title={Using next generation sequencing to identify yellow fever virus in Uganda.},
  author={L K McMullan and Mike Frace and Scott A. Sammons and Trevor R Shoemaker and Stephen Balinandi and Joseph Francis Wamala and Julius Julian Lutwama and R. Gregory Downing and Ute Stroeher and Adam Macneil and Stuart T Nichol},
  volume={422 1},
In October and November 2010, hospitals in northern Uganda reported patients with suspected hemorrhagic fevers. Initial tests for Ebola viruses, Marburg virus, Rift Valley fever virus, and Crimean Congo hemorrhagic fever virus were negative. Unbiased PCR amplification of total RNA extracted directly from patient sera and next generation sequencing resulted in detection of yellow fever virus and generation of 98% of the virus genome sequence. This finding demonstrated the utility of next… CONTINUE READING
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thankMike Flint for editing assistance and Serena Carroll for advice on phylogenetic analysis. We gratefully acknowledge the efforts of the Uganda VHF outbreak response

  • B. H. Bird, D. A. Bawiec, T. G. Ksiazek, T. R. Shoemaker, S. T. Nichol
  • 2007
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