• DOI:10.1111/age.12039
• Corpus ID: 5330093

Using molecular markers for pedigree reconstruction of the greater amberjack (Seriola dumerili) in the absence of parental information.

@article{RodriguezBarreto2013UsingMM,
  title={Using molecular markers for pedigree reconstruction of the greater amberjack (Seriola dumerili) in the absence of parental information.},
  author={Deiene Rodriguez-Barreto and Sofia Consuegra and Salvador J{\'e}rez and Juana Rosa Cejas and V Mart{\'i}n and Antonio Lorenzo},
  journal={Animal genetics},
  year={2013},
  volume={44 5},
  pages={
          596-600
        }
}

• D. Rodriguez-Barreto, S. Consuegra, +3 authors A. Lorenzo
• Published 1 August 2013
• Biology
• Animal genetics

Ensuring appropriate levels of genetic diversity in captive populations is essential to avoid inbreeding and loss of rare alleles by genetic drift. Pedigree reconstruction and parentage analysis in the absence of parental genotypes can be a challenging task that relies in the assignment of sibship relationships among the offspring. Here, we used eight highly variable microsatellite markers and three different assignment methods to reconstruct the most likely genotypes of a parental group of wild Seriola dumerili fish based on the genotypes of six cohorts of their offspring, to assess their relative contributions to the offspring. We found that a combination of the four most variable microsatellites was enough to identify the number of parents and their contribution to the offspring, suggesting that the variability of the markers can be more critical than the number of markers. Estimated effective population sizes were lower than the number of breeders and variable among years. The results suggest unequal parental contribution that should be accounted for breeding programs in the future.