Using liquid crystals as a readout system in urinary albumin assays.

@article{Alio2011UsingLC,
  title={Using liquid crystals as a readout system in urinary albumin assays.},
  author={Vera Joanne Ali{\~n}o and Kun-Lin Yang},
  journal={The Analyst},
  year={2011},
  volume={136 16},
  pages={
          3307-13
        }
}
Detecting human serum albumin (HSA) in urine samples is a standard procedure for the diagnosis of kidney problems and the prognosis of patients with chronic kidney diseases (CKDs). In this study, we developed a HSA assay by incorporating a thin layer of liquid crystals (LCs) as a readout system such that the presence of HSA in urine samples can be detected as optical signals. In combination with dilution protocols, this assay can be used to estimate the concentration range of HSA simply by… Expand
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Liquid crystal-based immunoassay for detecting human serum albumin
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This paper presents a wireless immunoglobulin-coated magnetoelastic (ME) biosensor for fast, cost-effective detections of human serum albumin (HSA) with small sample volumes at a microliter scale and shows high potential to be used in diagnosing abnormal HSA. Expand
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A novel single-site, double-sensing fluorescent probe 1 is reported, based on the finding that HSA can catalyze a Michael addition reaction between the probe and SO2, which induces a change in fluorescence, which may provide a significant boost in the study of the role of HSA in medicine and pharmacy. Expand
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TLDR
A novel hydrazide Schiff base fluorescent probe N'-((7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)pyrazine-2-carbohydrazide (NPC), which self-assembled into nanoparticles in aqueous solution, which exhibited superior selectivity and sensitivity toward HSA. Expand
Red-emitting benzo[e]indolium probes for HSA based on the TICT characteristics
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References

SHOWING 1-10 OF 34 REFERENCES
Chip electrophoresis as a method for quantifying total microalbuminuria.
TLDR
Compared with immunoassay, the chip electrophoresis identified higher microalbuminuria concentrations in all urine samples and clearly resolved the albumin peak from interfering proteins. Expand
On-site quantification of human urinary albumin by a fluorescence immunoassay.
TLDR
A fluorescence immunochromatography assay (ICA) for quantitative determination of albumin in urine and adopts the inherent simplicity of a lateral-flow ICA and uses a competitive immunoassay mode with a simple, one-step operation. Expand
Development and validation of a particle-enhanced turbidimetric inhibition assay for urine albumin on the Dade aca analyzer.
TLDR
A particle-enhanced immunoturbidimetric inhibition assay for urine albumin on the Dade aca analyzer is developed, which removes any of the potential antigen excess difficulties that could be expected from the wide clinical range of urine album in patients with diabetes mellitus. Expand
Laser immunonephelometry for routine quantification of urinary albumin excretion.
TLDR
A laser-immunonephelometric method for quantifying urinary albumin excretion (UAE) in large numbers of samples correlated well with those obtained by a RIA method, and one can assay 240 samples per hour. Expand
Quantitative determination of low and high molecular weight proteins in human urine: influence of temperature and storage time.
Many authors have studied the influence of storage on measured albumin concentrations in urine. Whereas some authors report that albumin concentrations decrease upon storage of urine at −20 °C… Expand
Differences in urinary albumin detected by four immunoassays and high-performance liquid chromatography.
TLDR
There is no global standard by which urinary albumin assays may be standardized, and this study suggests the need for such standards. Expand
Which method for quantifying “microalbuminuria” in diabetics?
TLDR
ITA seems suitable for initial screening of albuminuria in diabetic patients but more sensitive procedures (such as RIA and INA) seem preferable for measurement of UA concentrations in the normal range. Expand
Assessment of immunochemical methods for determining low concentrations of albumin in urine.
TLDR
Four immunochemical methods for measuring urinary albumin at low concentrations were assessed for their assay characteristics and practicability and capital cost was highest with RIA and lowest with RID, which required most technical skill. Expand
Immunoturbidimetry: an attractive technique for the determination of urinary albumin and transferrin.
TLDR
Two immunoturbidimetric assays for the measurement of urinary albumin and transferrin which are adapted for the Cobas-BIO centrifugal analyzer were developed and established using urines from 17 healthy individuals. Expand
Diagnostic potential of urine proteome: a broken mirror of renal diseases.
TLDR
An overview of studies into the urine proteome illustrates its potential value for diagnostic, prognostic, and pathophysiologic discovery and the idea that signature profiles of injury to individual nephron segments can be measured by this technology. Expand
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