Using green fluorescent protein to study intracellular signalling.

  title={Using green fluorescent protein to study intracellular signalling.},
  author={Jeremy Tavare and Laura M Fletcher and Gavin I. Welsh},
  journal={The Journal of endocrinology},
  volume={170 2},
Subcellular compartmentalisation of signalling molecules is an important phenomenon not only in defining how a signalling pathway is activated but also in influencing the desired physiological output of that pathway (e.g. cell growth or differentiation, regulation of metabolism, cytoskeletal changes etc.). Biochemical analyses of protein and lipid compartmentalisation by, for example, subcellular fractionation presents many technical difficulties. However, this aspect of cell signalling… CONTINUE READING
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