Using fluorescence for improvement of the quantitative analysis of micronucleus in cell culture.

@article{Dias2005UsingFF,
  title={Using fluorescence for improvement of the quantitative analysis of micronucleus in cell culture.},
  author={Viviane Miranda Dias and Renata Manelli-Oliveira and Gl{\'a}ucia Maria Machado-Santelli},
  journal={Mutation research},
  year={2005},
  volume={565 2},
  pages={
          173-9
        }
}
The micronucleus test is of unquestionable importance, being widely used in studies with several purposes. Standardization of the technique has been proposed by several groups mainly when in vivo assays are performed. In cell cultures the staining method that predominates is the Feulgen's reaction, since it is specific for DNA. In this work, we evaluated the use of two fluorescent stains, SYTOX green and propidium iodide, in substitution for the Feulgen's reaction. Based on the results we… CONTINUE READING

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