Using environmental DNA to assess population‐wide spatiotemporal reserve use

  title={Using environmental DNA to assess population‐wide spatiotemporal reserve use},
  author={Kathryn A. Stewart and Hongju Ma and Jinsong Zheng and Jianfu Zhao},
  journal={Conservation Biology},
Scientists increasingly rely on protected areas to assist in biodiversity conservation, yet the efficacy of these areas is rarely systematically assessed, often because of underfunding. Still, adaptive management strategies to maximize conservation success often rely on understanding the temporal and spatial dynamism of populations therein. Examination of environmental DNA (eDNA) is a time and cost‐effective way to monitor species’ distribution, and quantitative polymerase chain reaction (qPCR… 

Comparing conservation monitoring approaches: traditional and environmental DNA tools for a critically endangered mammal

While conservation management has made tremendous strides in the last few decades, the decision of knowing where and how to invest (often) small surveying budgets for biodiversity data collection

Evaluating monitoring options for conservation: comparing traditional and environmental DNA tools for a critically endangered mammal

The results suggest cPCR as the least expensive sampling option but the lack of PCR sensitivity suggests it may not be the most robust method for this taxon, predominately useful as a supplementary tool or with large expected populations.

Water, water everywhere: environmental DNA can unlock population structure in elusive marine species

A novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples is developed, exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing.

Enhancing tropical conservation and ecology research with aquatic environmental DNA methods: an introduction for non‐environmental DNA specialists

Biodiversity conservation is a worldwide concern and proper management of threatened species or communities depends on reliable and accurate data collection. Despite the broad utility of

Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

This work reviews and synthesizes eDNA studies published to date to highlight the opportunities and limitations of utilizing eDNA in ecology and conservation, and identifies potential ways of reducing limitations in eDNA analysis.

Understanding the effects of biotic and abiotic factors on sources of aquatic environmental DNA

  • K. Stewart
  • Environmental Science
    Biodiversity and Conservation
  • 2019
A review of publications on aquatic macroorganism eDNA that have evaluated or considered the effect of sources on signal detection (or quantification) and finds few studies acknowledge, and fewer still evaluate, the impact of eDNA production on genomic signal recovery.

Environmental DNA analysis as an emerging non-destructive method for plant biodiversity monitoring: a review

Abstract Environmental DNA (eDNA) analysis has recently transformed and modernized biodiversity monitoring. The accurate detection, and to some extent quantification, of organisms

Reinforcement of Environmental DNA Based Methods (Sensu Stricto) in Biodiversity Monitoring and Conservation: A Review

The investigation shows that the eDNA technique is applicable largely in early detection of invasive species, species detection for conservation, community-level biodiversity monitoring, ecosystem health monitoring, and study on trophic interactions, etc, and shows a great promise with its high accuracy and authenticity.

Investigating the distribution of the Yangtze finless porpoise in the Yangtze River using environmental DNA

Although YFP was visually observed in the Yangtze River in winter, water samples collected during the summer contained significantly higher YFP eDNA than winter water samples, and the eDNA-based method had higher detection rates than traditional field survey methods.

The effects of spatial and temporal replicate sampling on eDNA metabarcoding

The results indicate that small-scale spatial and temporal variation in eDNA groups might be susceptible to large community shifts in relatively short periods of time, highlighting the need to take temporal variations into consideration when assessing their usability as water quality indicators.



Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal

The impact primer design may have on eDNA applications in general and future considerations for conservation efforts with the Yangtze finless porpoise are summarized and suggested.

Monitoring endangered freshwater biodiversity using environmental DNA.

It is demonstrated that entire faunas of amphibians and fish can be detected by high-throughput sequencing of DNA extracted from pond water, underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for monitoring rare and threatened species across a wide range of taxonomic groups.

Development and Validation of Environmental DNA (eDNA) Markers for Detection of Freshwater Turtles

It is found that eDNA from turtles can be detected using both conventional polymerase chain reaction (PCR) and quantitative PCR (qPCR), and that the cost of detection through traditional survey methods is 2–10X higher than eDNA detection for the species in the study range.

Investigating the Potential Use of Environmental DNA (eDNA) for Genetic Monitoring of Marine Mammals

To determine the potential use of eDNA for genetic monitoring, specific primers that amplify short mitochondrial DNA sequences were used to detect the presence of a marine mammal, the harbor porpoise, in a controlled environment and in natural marine locations.

An environmental DNA‐based method for monitoring spawning activity: a case study, using the endangered Macquarie perch (Macquaria australasica)

It is shown that changes in the relative abundance of nuclear and mitochondrial eDNA can be used to monitor spawning activity of the endangered Macquarie perch and is likely to be transferrable to other aquatic species.

Environmental conditions influence eDNA persistence in aquatic systems.

Measurements of local environmental conditions, consideration of environmental influence on eDNA detection, and quantification of local eDNA degradation rates will help interpret future eDNA surveillance results.

Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples

Environmental DNA (eDNA) methods for detecting aquatic species are advancing rapidly, but with little evaluation of field protocols or precision of resulting estimates. We compared sampling results