Using chimeric hypoviruses to fine-tune the interaction between a pathogenic fungus and its plant host.

@article{Chen2000UsingCH,
  title={Using chimeric hypoviruses to fine-tune the interaction between a pathogenic fungus and its plant host.},
  author={Baoshan Chen and Lynn M. Geletka and Donald L. Nuss},
  journal={Journal of virology},
  year={2000},
  volume={74 16},
  pages={7562-7}
}
Infectious cDNA clones of mild (CHV1-Euro7) and severe (CHV1-EP713) hypovirus strains responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica were used to construct viable chimeric viruses. Differences in virus-mediated alterations of fungal colony morphology, growth rate, and canker morphology were mapped to a region of open reading frame B extending from nucleotides 2,363 to 9, 904. By swapping domains within this region, it was possible to… CONTINUE READING

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